Taq DNA 聚合酶
由于Taq DNA聚合酶没有3‘至5’的外切酶活性,因此在DNA聚合过程中相当于核苷酸转移酶的作用,最终会导致PCR产物的3‘末端会产生3’-dA overhangs,即产生带一个A的3’粘端。本Taq DNA聚合酶由大肠杆菌表达纯化得到。
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